Journal: Toxics

Article Title: Ferulic Acid Derivatives Ameliorate Intestine Barrier Destruction by Alleviating Inflammatory Responses in Dextran Sulfate Sodium-Induced Inflammatory Bowel Disease

doi: 10.3390/toxics12040268

Figure Lengend Snippet: Anti-inflammatory effect of FA derivatives C1 and C1a in macrophages: ( a ) the cytotoxicity of FA, C1, and C1a was determined on J774A.1 cells and mouse peritoneal macrophages; ( b ) LPS-stimulated nitric oxide production was determined using a nitric oxide detection kit on J774A.1 cells and mouse peritoneal macrophages; ( c ) the LPS-stimulated expression of pro-inflammatory cytokines and mediators including IL-1β, IL-6, MCP-1, iNOS, and COX-2 in J774A.1 cells was analyzed by qPCR; ( d ) the LPS-stimulated activation of intracellular mediators, including iNOS and COX-2, was confirmed by Western blot. ( e ) the LPS-stimulated phosphorylation of NF-κB was assessed by ELISA. Data are means ± SEM of three independent experiments. Note: # p < 0.05 is a significant difference compared with control group; * p < 0.05 is a significant difference compared with LPS-treated group.

Article Snippet: The phosphorylation of nuclear factor (NF)-κB in LPS-stimulated J774A.1 cells was determined with an ELISA kit (#7834S; Cell Signaling Technology) according to the manufacturer’s protocol.

Techniques: Expressing, Activation Assay, Western Blot, Phospho-proteomics, Enzyme-linked Immunosorbent Assay, Control

Journal: Artificial cells, nanomedicine, and biotechnology

Article Title: Silence of lncRNA MIAT protects ATDC5 cells against lipopolysaccharides challenge via up-regulating miR-132.

doi: 10.1080/21691401.2019.1626410

Figure Lengend Snippet: Figure 4. Suppressive effect of MIAT silence on the activation of NF-jB and JNK pathways via miR-132. ATDC5 cells were co-transfected with sh-MIAT and miR-132 inhibitor and then subjected to 6 lg/mL LPS. The phosphor/total (p/t) levels of (A,B) p65, IjBa and (C,D) JNK were measured using western blot (n ¼ 3). p < .05 (ANOVA combined with Duncan post-hoc test).

Article Snippet: Anti-caspase-3 (orb378617), anti-cleavedcaspase-3 (orb106556), anti-caspase-9 (orb135175), anticleaved-caspase-9 (orb227889), anti-PARP (orb526607), anti-cleaved-PARP (orb106557), anti-IL-6 (orb6210), anti-IL-8 (orb39299), anti-TNF-a (orb475251), anti-MCP-1 (orb97456), anti-p65 (orb344389), anti-p-p65 (orb14753), anti-IjBa (orb338946), anti-p-IjBa (orb99312), anti-JNK (orb38050), anti-p-JNK (orb184488), and anti-b-actin (orb86987) all from Biorbyt (San Francisco, CA).

Techniques: Activation Assay, Transfection, Western Blot